LOCUS       pLV-[hsa-miR-924]        9107 bp    DNA     circular     27-JUN-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7028..7888
                     /label=Amp(R)
     promoter        8089..8629
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5633..5867
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6442..6897)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5939..6069
                     /label=SV40 pA
     misc_feature    6070..6283
                     /label=SV40 ori
     misc_feature    4954..5550
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3572
                     /label=intron
     CDS             3619..4944
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3362
                     /label=hsa-mir-924 precursor
BASE COUNT     2158 a      2370 c      2447 g      2132 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatccc tttctgcaat 
     3121 caaactaaac cagtttctgt tatttatcct aagatagatt gctggtgaaa attcctcaac 
     3181 tgaccaccca cagaccacct aagccagtca atagagtctt gtgatgtctt gcttaagggc 
     3241 catccaacct agagtctaca actgattccc ttccatccta tggtttttgc ctttataata 
     3301 attttctact ccttgactcc tcttcggtgt gtattttggt ttgcactgaa ggctgcatct 
     3361 ccgctagctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga 
     3421 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt 
     3481 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga 
     3541 cagtggttca aagttttttt cttccatttc aggtgtcgtg aaaactaccc ctctagagtc 
     3601 gagctaccgg tcgccaccat ggtgagcaag ggcgaggagg ataacatggc catcatcaag 
     3661 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc 
     3721 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc 
     3781 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc 
     3841 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag 
     3901 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag 
     3961 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc 
     4021 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg 
     4081 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac 
     4141 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg 
     4201 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc 
     4261 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg 
     4321 tacaaggacc caccggtcgc caccatgacc gagtacaagc ccacggtgcg cctcgccacc 
     4381 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc 
     4441 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc 
     4501 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg 
     4561 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc 
     4621 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc 
     4681 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc 
     4741 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag 
     4801 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag 
     4861 cggctcggct tcaccgtcac cgccgacgtc gagtgcccga aggaccgcgc gacctggtgc 
     4921 atgacccgca agcccggtgc ctgagcggcc gcgtcgacaa tcaacctctg gattacaaaa 
     4981 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg 
     5041 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct 
     5101 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg 
     5161 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct 
     5221 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg 
     5281 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg 
     5341 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc 
     5401 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc 
     5461 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc 
     5521 ggatctccct ttgggccgcc tccccgcctg gaattcgagc tcggtacctt taagaccaat 
     5581 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg 
     5641 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt 
     5701 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca 
     5761 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa 
     5821 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg 
     5881 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa 
     5941 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 
     6001 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 
     6061 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc 
     6121 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag 
     6181 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg 
     6241 acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac 
     6301 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc 
     6361 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc 
     6421 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg 
     6481 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt 
     6541 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc 
     6601 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg 
     6661 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg 
     6721 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct 
     6781 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg 
     6841 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg 
     6901 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc 
     6961 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag 
     7021 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg 
     7081 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt 
     7141 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt 
     7201 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt 
     7261 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa 
     7321 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag 
     7381 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac 
     7441 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac 
     7501 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac 
     7561 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac 
     7621 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact 
     7681 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg 
     7741 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt 
     7801 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat 
     7861 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta 
     7921 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa 
     7981 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga 
     8041 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac 
     8101 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt 
     8161 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc 
     8221 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat 
     8281 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag 
     8341 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc 
     8401 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag 
     8461 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac 
     8521 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg 
     8581 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct 
     8641 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc 
     8701 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga 
     8761 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga 
     8821 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg 
     8881 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt 
     8941 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt 
     9001 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc 
     9061 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgc 
//
